Instructions to Candidates
- This paper consists of two sections A and B.
- Answer all the questions in Sections A in the spaces provided.
- You should check the question paper to ascertain that all the pages are printed as indicated and that no questions are missing.
SECTION A
Answer all the questions in Sections A in the spaces provided.
- Name the three major branches of biology. (3mks)
- Explain why the following processes are important during the preparation of temporary slides:-
- Staining (1mk)
- Use of a sharp cutting blade (1mk
- Distinguish between the following terms :-
- Magnification and resolution of a microscope (2mks)
- Mounting and staining of a specimen (2mks)
- The diagrams below show an experiment set up to investigate a certain process in a plant tissue.
Explain the results obtained after 30 min. (3mks) - A student mashed a piece of raw banana and made it into paste by adding water, placed the paste in a visking tubing and suspended it in a beaker containing iodine solution as shown below. The set- up was left for 40 minutes.
- State the physiological process under investigation. (1 mark)
- Account for the result obtained in the table. (2 marks)
- Arrange the following from the largest to the smallest:
Tissue,cell,organism,organelle,organ system,organ, ( 1mk) - State two factors that affect enzymatic activities (2 marks)
- Name the sites where light and dark reactions of photosynthesis take place. ( 2 mks)
- The diagram represents part of the human digestive system.
- Name the organs labeled L and M. (2mks)
-
- Name the juice secreted by K. (1mk)
- State the function of the juice named in (b) (i) above (3mks)
- Name one enzyme secreted by M (1mks)
-
- Name two salivary glands found in human mouth (2mks)
- state two functions of saliva (2mks)
-
- State two ways in which the guard cells differ from other adjacent epidermal cells 2mks)
- Apart from guard cells name other two cells that carryout photosynthesis in a leaf.(2mk)
- Give the importance of leaf mosaic in the process of photosynthesis. (1mk)
- State the functions of the following cell organelles. (2 mks
- Golgi apparatus
- Ribosomes
- The table below shows the concentration of important plant nutrients
ion Concentration in pond water (ppm) Concentration in cell sap (ppm) Potassium 200 50 Chloride 0.5 20 - Potassium
- Chloride
- State two properties of monosaccharide. (2mks)
SECTION B
- The diagram below illustrates the behavior of red blood cells when placed into two different solutions X and Y.
- Suggest the nature of solution X and Y (2mks)
- Name the process represented by each of the letters A and B (2mks)
- Explain the observation made at the end of process B above. (3mks)
- A plant cell was placed in solution Y, and it did not burst. State the structure in the plant cell that prevented it from bursting.(1mk)
- The figure below shows a diagram of a cell as seen under a light microscope. Study it and answer the questions that follow.
- Name three structures that show that it is a plant cell and not a animal cell 3mks
- Name the photosynthetic pigment that is found in the structure labelled Z and state its function. 2mks
- State the name and function of the fluid found in X 2mks
- On the diagram label the structure that contains the DNA 1mk
- A potted plant with healthy leaves was kept in the dark for 48 hours. One of the leaf was then partly enclosed in a wide boiling test tube containing sodium hydroxide solution. The whole set up was then kept in sunlight for six hours. The diagram is shown below.
- Suggest the aim of the experiment. (1mk)
- Why was the set up kept in the dark for 48 hours? (1mk)
- Why was the set up kept in the sunlight for six hours? (1mk)
- What is the importance of sodium hydroxide solution in the set up? (1mk)
- State the colours observed on each part when the leaf was tested for starch (3mks)
- State the form in which carbohydrates are stored in the liver. (1mk)
- The organisms below were collected from a certain habitat. Study them and answer the questions that follow.`
- In which kingdom do the organisms belong (1mk)
- State two external features that are found in organism B. 2mks
- State the method used to collect:
- Organism B 1mk
- Organism D 1mk
- State one precaution that should be taken when collecting organism A 1mk
- Identify two modes of locomotion in specimen B ( 2mks)
- The set-up below illustrates a procedure that was carried out in the laboratory with a leaf plucked from a green plant that had been growing in sunlight.
- What was the purpose of the above procedure? (1mark)
- Give reasons for carrying out step A,B and C in this procedure. 3marks)
- Name the reagent that was used at the step labeled D . (1mark)
- State the expected result on the leaf after adding the reagent named in (iii) above.(1mark)
- Describe the adaptations of the ileum to its function 20 marks

MARKING SCHEME
- Name the three major branches of biology.(3mks)
- Zoology
- Botany
- Biochemistry
- Explain why the following processes are important during the preparation of temporary slides :-
- Staining (1mk)
- Make cells visible;
- Use of a sharp cutting blade(1mk)
- Prevent distortion of cells;
- Staining (1mk)
- Distinguish between the following terms :-
- Magnification and resolution of a microscope (2mks)
- Magnification – Ability of a microscope to enlarge tiny objects
- Resolution – Ability of a microscope to separate between two tiny structures under magnification to appear distinct
- Magnification – Ability of a microscope to enlarge tiny objects
- Mounting and staining of a specimen (2mks)
- Mounting – The placing of prepared slide on stage of a microscope;
- Staining – Use of chemical stain on specimen for clear observation
- Magnification and resolution of a microscope (2mks)
- The diagrams below show an experiment set up to investigate a certain process in a plant tissue.
Explain the results obtained after 30 min.(3mks)- Water was hypotonic to cell sap of adjacent; and these cell absorb water through osmosis; and their cell sap became less conc. Than those of next cell; the process was repeated until water reached the sugar solution.;
Or - Sugar solution was hypotonic to cell sap of adjacent cells; they lost water by osmosis; cell sap became more conc. than those of next cell; the process was repeated until water was drawn from the beaker.
- Water was hypotonic to cell sap of adjacent; and these cell absorb water through osmosis; and their cell sap became less conc. Than those of next cell; the process was repeated until water reached the sugar solution.;
- A student mashed a piece of raw banana and made it into paste by adding water, placed the paste in a visking tubing and suspended it in a beaker containing iodine solution as shown below. The set- up was left for 40 minutes.
- State the physiological process under investigation. (1 mark)
- Diffusion
- Account for the result obtained in the table. (2 marks)
- Iodine molecules from the beaker moved into the visking tubing by diffusion since they were small in size; Color of iodine solution outside remained brown as starch molecules were too large to pass through the visking tubing. (2 marks)
- State the physiological process under investigation. (1 mark)
- Arrange the following from the largest to the smallest : Tissue,cell,organism,organelle,organ system,organ, ( 1mk)
- Organism,organ system,organ,tissue,cell,organelle;
- State two factors that affect enzymatic activities (2 marks)
- Temperature pH co- factors, co- enzymes; enzyme product concentration; substance concentration/ metabolic poison
- Name the sites where light and dark reactions of photosynthesis take place ( 2 mks)
- Light reaction - Granum/thylokoid
- Dark reaction - Stroma
- The diagram represents part of the human digestive system.
- Name the organs labeled L and M.(2mks)
- L - Duodenum
- M - Pancrease
-
- Name the juice secreted by K.(1mk)
- bile
- state the function of the juice named in (b) (i) above(3mks)
- emulsification/ emulsification of fat; neutralize acidic chime from stomach; provides alkaline media(for enzyme to work)
- Name the juice secreted by K.(1mk)
- Name one enzyme secreted by M (1mks)
- lipase; pancreatic amylase;trypsin
- Name the organs labeled L and M.(2mks)
-
- Name two salivary glands found in human mouth (2mks)
- Sublingual; submaxillary/ submandibular; parotid
- state two functions of saliva (2mks)
- Lubricating food; digestion of starch; moistens food; provides alkaline medium; soften food; dissolves food. Acc, for correct component of saliva to correct function
- Name two salivary glands found in human mouth (2mks)
-
- State two ways in which the guard cells differ from other adjacent epidermal cells (2mks)
- Guard cells have chloroplast;
- They are bean shaped;
- Apart from guard cells name other two cells that carryout photosynthesis in a leaf.(2mk)
- Spongy mesophyll cell;
- Palisade cells;
- State two ways in which the guard cells differ from other adjacent epidermal cells (2mks)
- Give the importance of leaf mosaic in the process of photosynthesis. (1mk)
- prevents some leaves from obstructing others from receiving sunlight
- State the functions of the following cell organelles.
- Golgi apparatus (2 mks
- Packaging and transport of glycoproteins
- Secretions of synthesised proteins.
- Ribosomes
- Manufacture / synthesis proteins
- Golgi apparatus (2 mks
- The table below shows the concentration of important plant nutrients
Name the process by which the above ions could have been taken up by the plants. 2mks)- Potassium- Diffusion
- Chloride-Active transport
- State two properties of monosaccharides.(2mks)
- Soluble in water;
- Sweet to taste;
- Are crystallisable;
- Are reducing sugars;
SECTION B
- The diagram below illustrates the behaviour of red blood cells when placed into two different solutions X and Y.
- Suggest the nature of solution X and Y (2mks)
- Solution X – hypertonic;
- Solution Y – hypotonic;
- Name the process represented by each of the letters A and B (2mks)
- Process A Crenation;
- Process B – Haemolysis;
- Explain the observation made at the end of process B above. (3mks)
- Red blood cell gains excess water by osmosis; from hypotonic solution; swell and burst ;
- a plant cell was placed in solution Y, and it did not burst. State the structure in the plant cell that prevented it from bursting.(1mk)
- cellulose cellwall/cell wall
- Suggest the nature of solution X and Y (2mks)
- The figure below shows a diagram of a cell as seen under a light microscope. Study it and answer the questions that follow.
- Name three structures that show that it is a plant cell and not a animal cell 3mks
- sap vacuole;cell wall;chloroplast;tonoplast
- Name photosynthetic pigment that is found in the structure labelled Z and state its function. 2mks
- Name: Chlorophyll
- Function: Traps light energy for photosynthesis
- state the name and function of the fluid found in X 2mks
- Name: Cell sap;
- Function: storage of salts and sugars
- On the diagram label the structure that contains the DNA 1mk
- Label the nucleus
- Name three structures that show that it is a plant cell and not a animal cell 3mks
- A potted plant with healthy leaves was kept in the dark for 48 hours. One of the leaf was then partly enclosed in a wide boiling tube containing sodium hydroxide solution. The whole set up was then kept in sunlight for six hours. The diagram is shown below.
- Suggest the aim of the experiment. (1mk)
- To show that carbon (IV) oxide is necessary for photosynthesis Or To show that light is necessary for photosynthesis
- Why was the set up kept in the dark for 48 hours? (1mk)
- to destarch the leaf
- Why was the set up kept in the sunlight for six hours? (1mk)
- to allow photosynthesis to take place
- What is the importance of sodium hydroxide solution in the set up? (1mk)
- to absorb carbon (IV) oxide
- State the colours observed on each part when the leaf was tested for starch (3mks)
- J Blue black
- K Brown
- L Brown
- State the form in which carbohydrates are stored in the liver. (1mk)
- glycogen;
- Suggest the aim of the experiment. (1mk)
- The organisms below were collected from a certain habitat. Study them and answer the questions that follow.`
- In which kingdom do the organisms belong 1mk
- Animalia;
- State two external features that are found in organism B. 2mks
- legs;wings;antennae;body segments;
- State the method used to collect:
- Organism B 1mk
- sweep net;
- Orgarnism D 1mk
- pitfall trap;
- Organism B 1mk
- State one precaution that should be taken when collecting orgarnism A 1mk
- should be handled with a pair of forceps
- Identify two modes of locomotion in specimen B ( 2mks)
- flying;walking;
- In which kingdom do the organisms belong 1mk
- The set-up below illustrates a procedure that was carried out in the laboratory with a leaf plucked from a green plant that had been growing in sunlight.
- What was the purpose of the above procedure ? (1mark)
- Testing (a leaf) the presence of starch
- Give reasons for carrying out step A,B and C in this procedure. (3marks)
- A. kill the leaf /cells/ protoplasm/ breakdown starch granules/ stop enzymatic activity
- B. Removal of chlorophyll/ dissolve chlorophyll /decolourise the leaf
- C. Soften leaf / makes less brittle.
- Name the reagent that was used at the step labeled D . (1mark)
- Iodine solution
- State the expected result on the leaf after adding the reagent named in (iii) above. (1mark)
- Stain dark blue/ Blue black
- What was the purpose of the above procedure ? (1mark)
- Describe the adaptations of the ileum to its function 20 marks
- Has secretory glands / crypts of lieberkuhn; which secretes enzymes; (maltase / sucrose / peptidase / lipase to complete digestion; of lipids / sugar / proteins.
- Goblet cells secrete mucus ;allows for smooth movement of food ; protect wall of ileum from action of digestive enzymes;
- Very long ;to provide large surface area for absorption;
- Highly folded / coiled; to slow movement of food to allow more time for digestion / absorption / increase surface area for absorption.;
- Has numerous villi ;which increase surface area for absorption / microvillus which further increase surface area for absorption.;
- Ileum wall / villi have thin epithelium; which is only one cell thick to reduce distance over which digested food has to diffuse;.
- Villi are highly vascularised / have a rich network of blood capillaries; for rapid transport of digested food from small intestines / maintain a steep concentration gradient.;
- Villi have lacteals ;for absorption of fatty acids and glycerol;
- Cells of ileum wall have a large count of mitochondria; to release energy that aid in active transport across the epithelium.; Max 20mks
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